DNA Vectors
Vector Biolabs GCaMP6m Adenovirus (Ad-GCaMP6m)
GCaMP is a genetically encoded calcium indicator (GECI) which was created from a fusion of green fluorescent protein (GFP), calmodulin, and M13, a peptide sequence from myosin light chain kinase. GECI binds Ca2+ and induce a change in fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ fluxes. The advantage of GECI's are that they can be genetically specified for studies in living organisms.The new novel GCaMP6 have increased ΔF/F0 and faster kinetics compared to previous GCaMP3 and GCaMP5G.This adenovirus expresses GCaMP6m.
Vector Biolabs High-Mobility Group Box 2 Adenovirus (Ad-HMG2)
High mobility group (HMG) proteins are non-histone DNA-binding proteins that induce alterations in the DNA architecture, including DNA bending and unwinding of the helix. Although HMG proteins may bind to single-stranded DNA, conformational changes are only induced in double-stranded DNA. HMG proteins synergize with Oct-2, members of the NFkB family, ATF-2, c-Jun and members of the steroid class of receptors to activate transcription.
Vector Biolabs human RB shRNA Adenovirus (Ad-h-RB-shRNA)
This is an pre-made gene silencing adenovirus that expresses a shRNA to knockdown human RB gene. The shRNA expression is driven by an U6 promoter.
The knockdown of this human gene was validated by western blot in HCT-116 cells.
Vector Biolabs Carnitine O-octanoyltransferase Adenovirus (Ad-CROT)
Carnitine octanoyltransferase is a carnitine acyltransferase that catalyzes the reversible transfer of fatty acyl groups between CoA and carnitine. This provides a crucial step in the transport of medium- and long-chain acyl-CoA out of the mammalian peroxisome to the cytosol and mitochondria.
Vector Biolabs AAV-CAG-GFP (AAV serotype 5) AAV (AAV5-CAG-GFP)
The CAG promoter is a hybrid of the cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter. The CAG promoter is used to drive high levels of gene expression in mammalian expression vectors.This AAV serotype 5 virus (Capsid from AAV5 and ITR from AAV2) expresses eGFP (Green Fluorescent Protein) under the control of CAG promoter.
Amsbio LLC CAR negative control: Anti-CD19-ScFv-4-1BB (Puro)
CAR negative control: Anti-CD19-ScFv-4-1BB
Vector Biolabs B-cell CLL/lymphoma 2 Adenovirus (Ad-BCL2)
Members of the Bcl-2 family of proteins interact to regulate programmed cell death (apoptosis). Various homodimers and heterodimers formed by proteins in this family either promote or inhibit apoptosis. Pro-apoptotic members of this family include Bax, Bcl-xS, Bad, Bak, NBK (Bik), BID, Hrk, Bok, Bim, NOXA, Diva and Bmf. Apoptotic inhibitors include Bcl-2, Bcl-xL, Bcl-x(beta), Bcl-x(gamma), Bcl-w, Mcl-1, Bag-1, A1 (Bfl-1), BAR and BI-1 (TEGT). Bok, or Bcl-2 related ovarian killer, induces apoptosis in cells, but is suppressed by anti-apoptotic Bcl-2 family proteins. BECN1 plays a significant role in autophagy. PUMA gene encodes two BH3 domain-containing proteins PUMA(alpha) and PUMA(beta), which have similar binding activities to Bcl-2, localize to the mitochondria to induce cytochrome c release and activate the rapid induction of programmed cell death.
Vector Biolabs aryl hydrocarbon receptor nuclear translocator Adenovirus (Ad-HIF1b/ARNT)
The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1)
Vector Biolabs AAV-Cre (AAV serotype 2) AAV (AAV2-Cre)
This AAV serotype 2 virus (both Capsid & ITR from AAV2) expresses Cre Recombinase. This Cre contains a nuclear localization signal (NLS).Cre recombinase is a Type I topoisomerase from P1 bacteriophage that catalyzes site-specific recombination of DNA between loxP sites. loxP is a 34 bp DNA sequence at which confers directionality. Cre recombinase is used as a tool to genetically modify genes, such as to delete a segment of DNA flanked by LoxP sites in cultured cells or experimental animals.
Vector Biolabs WEE1 homolog Adenovirus (Ad-WEE1)
Phosphorylation of Cdc2 on threonine 14 and tyrosine 15 is required to maintain Cdc2 in an inactive state throughout the S and G2 phases of the cell cycle. The human Wee 1 protein, Wee 1 Hu, encodes a tyrosine-specific protein kinase that phosphorylates Cdc2 on tyrosine 15. Myt 1, a member of the Wee 1 family of protein kinases, has been shown to phosphorylate Cdc2 on both threonine 14 and tyrosine 15 in a cyclin-dependent manner. Both Wee 1 Hu and Myt 1 activity are regulated during the cell cycle, suggesting that both proteins play a role in mitotic control. Dephosphorylation of Cdc2 on threonine 14 and tyrosine 15 in late G2 by Cdc25 then activates the Cdc2/cyclin B complex to allow entry into mitosis.